Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in Pastoral Regions of Ethiopia
نویسندگان
چکیده
A cross sectional study was conducted on 906 apparently healthy camels slaughtered at Akaki and Metehara abattoirs to investigate the pathology of camel tuberculosis (TB) and characterize its causative agents using postmortem examination, mycobacteriological culturing, and multiplex polymerase chain reaction (PCR), region of difference-4 (RD4)-based PCR and spoligotyping. The prevalence of camel TB was 10.04% (91/906) on the basis of pathology and it was significantly higher in females (χ(2) = 4.789; P = 0.029). The tropism of TB lesions was significantly different among the lymph nodes (χ(2) = 22.697; P = 0.002) and lung lobes (χ(2) = 17.901; P = 0.006). Mycobacterial growth was observed in 34% (31/91) of camels with grossly suspicious TB lesions. Upon further molecular characterization using multiplex PCR, 68% (21/31) of the colonies showed a positive signal for the genus Mycobacterium, of which two were confirmed Mycobacterium bovis (M. bovis) by RD4 deletion typing. Further characterization of the two M. bovis at strains level revealed that one of the strains was SB0133 while the other strain was new and had not been reported to the M. bovis database prior to this study. Hence, it has now been reported to the database, and designated as SB1953. In conclusion, the results of the present study have shown that the majority of camel TB lesions are caused by mycobacteria other than Mycobacterium tuberculosis complex. And hence further identification and characterization of these species would be useful towards the efforts made to control TB in camels.
منابع مشابه
Tuberculosis in Goats and Sheep in Afar Pastoral Region of Ethiopia and Isolation of Mycobacterium tuberculosis from Goat
A cross sectional study was conducted on 2231 small ruminants in four districts of the Afar Pastoral Region of Ethiopia to investigate the epidemiology of tuberculosis in goats and sheep using comparative intradermal tuberculin skin test, postmortem examination, mycobacteriological culture and molecular typing methods. The overall animal prevalence of TB in small ruminants was 0.5% (95% CI: 0.2...
متن کاملStudy on mange mite of camel in Raya-Azebo district, northern Ethiopia
A cross-sectional study was carried out to determine the prevalence and species of camel mange mite infestation in Raya-Azebo district, Northern part of Ethiopia. Accordingly, Three hundred and eighty-four camels were examined and mange mite infestation was detected on 64 of camels. Only Sarcoptes scabiei var. cameli was identified as the only mite species in all skin...
متن کاملIsolation and molecular characterization of partial FSH and LH receptor genes in Arabian camels (Camelus dromedarius)
Very little is known about LHR and FSHR genes of domestic dromedary camels. The main objective of this study was to determine and analyze partial genomic regions of FSHR and LHR genes in dromedary camels for the first time. To this end, a total of 50 DNA samples belonging to dromedary camels raised in Iran were sent for sequencing (25 samples of each gene). We compared the nucleotide sequences ...
متن کاملMolecular Characterization of Echinococcus granulosus Isolated from Sheep and Camel in Iran
متن کامل
Analysis of Factors Determining Households Food Security in Pastoral Area Oromia Region, Moyale District, in Ethiopia
This study examined the status and determinants of rural households’ food security in Moyale district of pastoral area, Oromia regional state, in Ethiopia. Both secondary and primary data were used for the study. The study was based on the survey of a total of 100 farmers randomly selected using a three-stage sampling technique. Analytical tools used include descriptive statistics, Foster-Greer...
متن کامل